Particle discriminator interface for nanoflow ESI-MS

Variations in plasma and urinary lipids in response to enzyme replacement therapy for Fabry disease patients by nanoflow UPLC-ESI-MS/MS.

A deficiency of α-galactosidase A causes Fabry disease (FD) by disrupting lipid metabolism, especially trihexosylceramide (THC). Enzyme replacement therapy (ERT) is clinically offered to FD patients in an attempt to lower the accumulated lipids. Studies on specific types of lipids that are directly or indirectly altered by FD are very scarce, even though they are crucial in understanding the bi...

Comparison of HPLC-Chip/MS with conventional nanoflow LC/MS for proteomic analyses

Nanoflow LC/MS is commonly used for protein identification and biomarker discovery, but setting up and maintaining the intricate LC plumbing can be a challenge. The Agilent HPLC-Chip represents breakthrough technology that integrates nanoflow HPLC columns, associated connections, and a nanoelectrospray emitter on a single, small, reusable microfluidic chip. The HPLC-Chip and a companion HPLCChi...

ESI and nanoESI - CID - MS / MS

Nanoflow LC/IMS-MS and LC/IMS-CID/MS of protein mixtures.

A simple ion trap/ion mobility/time-of-flight (TOF) mass spectrometer has been coupled with nanoflow liquid chromatography to examine the feasibility of analyzing mixtures of intact proteins. In this approach proteins are separated using reversed-phase chromatography. As components elute from the column, they are electrosprayed into the gas phase and separated again in a drift tube prior to bei...

LC-ESI-MS/MS Alkaloidal Profiling and Biological Investigation of Cleistocactus winteri Stems

Cleistocactus winteri is a succulent plant of the Cactaceae family, commonly known as the golden rat tail. Many members of the Cactaceae family are the focus of chemical and biological studies as they contain bioactive compounds, well known for their health-related properties. We aimed to investigate Cleistocactus winteri stems both phytochemically and biologicallyfor the firs...